900612 ESP -- SURF Center : G3 -Turbine- Technical Options

This page last changed on Oct 08, 2012 by pargett.

	G3 Function	Requirements	Technologies / Development needs	Manufacturers/ Collaborators	Score*
1	Ambient Flush Reservoir	Mixtures, chemicals? Volume (s) Material compatibility Dilution with SW? Flush fluids as well?			1
2	Intake point and Screen	Single intake? CU screens? Location on LRAUV Min bore size?			2
3
4	Intake Selector Valve Ambient Sea/Flush	Number of ports (intakes and ambient storage)? 3-way valve Min bore size? pressure neutral 15 psid	Solenoid oil compensated multi port flipper style Rocker style		3
5	Bulkhead valves	Isolation of seawater path Up to 500 psi Min bore size? Non-corroding Fail closed Wattage? Power budget? Small scale, Size: less than c-battery	Direct lift solenoid valves Custom body valves (thread into a SAE 4 port) Axial flow thru valves Turn or axial pull valves Multi port valves Automated actuators of above valves.		3
6	Cartridge Selection valves	Chemical suitable 500 psi Min bore size? low power flush free	Ladder/Looper options 2 position spool valves Direct lift solenoid valves Custom body valves 1/4 Turn Axial pull Automated actuators of above valves. Multiple bank valves integrated on a manifold	In house design, manifold w/ Axial pull spool valve 1/4 turn	4
7	Cartridge Return Array		check valve part of Selection valve	LeeCo Parker	4
8	Sea Pressure fittings/Tubing	500 psi 1mm min thru sea water and reagent stable vibration resistant reliable seal	1/4-28 flat bottom fittings Testing process, procedure Vibration testing?	Dibafit Upchurch LeeCo	1
9	Filter holder sealing	500 psi sea water and reagent stable vibration resistant reliable seal Seal validation prior to filtering			2
10	Filter selection valving (transition from sea to reagents)	500 psi sea water and reagent stable vibration resistant reliable seal	Rotary valve, reagent select, part of filter holder		4
11	Cartridge Seals	Connection between spool valves, cartridges to cartridge			2
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14	Filter Media	25mm dia (effective) area	Durapore Supore		1
15	Filter Pumping	75 psi (5 bar bubble point) Variable speed Max flow at least 4L/hr (@22psi) Min flow 300mL/hr Dosing accuracy <1% Positive displacement (or flow measuring) seawater suitable Minimal shear damage to microbes. Bidirectional (not required, but valuable) Size (with drive mech, but not motor) < soda can	Piston pumps Rotating piston (Similar to DWSM) Syringes (with or without flow valves) Gear pumps (low expectation) Peristaltic (low expectation) Progressive cavity (typically large frame) Diaphragm Others?		3
16	Filter Evacuate	Need to remove seawater <5% of lysis volume. In G2, remaining seawater needs to be <100uL For 1mL lysis volume, <50uL, or <1 drop. Test numbers: Dry filter +holder, 9.91g wet filter (filter holder dry) 9.95g (40 uL) no change if vacuumed, no change w/ singe 10ml blow thru after 3X 10mL blow thru, 9.92g (10 uL) filter appeared to be mostly dry	Air pressure evac (G2 method) Vacuum assist below filter? Filter holder is piston assembly, w/ small dead volumes Displacement with other fluid?		4
17	Lyse	Up to 1mL of buffer w/ w.o beads Sample particles on filter Qiagen Lysis buffer RLT, RLT+, >RNA & DNA SPR buffer Aq Methanol	Bead Beating Heating Integrated heater/filter fit or holder Grinding Magnetic driven beating/grinding Acoustic/Sonic Sequential Chemistry Automation of the above in closed volumes	DNA/RNA: (4) Microsonic (8/10 lyse) w/ w.o beads (4) Magnet flipper (4) Bead Mill Grinder (2) Heat (5/10 lyse) Ref Bead Beat (10/10 lyse)DA/rRNA: (2) Heat	4
18	DNA/RNA purification	Purification of 500-1000uL of lysate RNA and DNA in single sample desired.	RNA and DNA extraction from one sample Magnetic bead Need sources, size and coating Glass bead/fiber columns Non-centrifuge wash processes. Automated systems that perform the above.	(3) Qiagen Membrane Single use only Mock up on MFB May need a pressure sensor (4) iFast May or may not be renewable Currently using RLT For DA: pass beads to SPR For NA: no beads pass (3) Current silica column Renewable (3) Chip Shop Mag bead	3
19	Direct Capture
20	Reagent storage	Bulk storage to 500mL Single use storage 50uL to 1mL	Blister packs Prefilled syringes Imbedded syringes Lyophilized Dry powder (beads)		4
21	Reagent dosing	Deliver 50-750uL doses of liquid reagent Deliver single shot reagent doses Deliver/dose powder (beads)	Compressed gas systems Linear or cam actuators of blisters/syringes Actuation methods for any of the above storages, such as Dissolving/melting pills or isolators.		4
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23	Rotating Assembly	Spindle, supporting 30+ cartridges Structurally supports actuator forces Support g loads Retains cartridges against g loads, pressure Thur bore sufficient for wiring harnesses Mech should sin freely, but operate +180/-180	Cantilever spindle large diameter alignment bearing Between bearings (req's end support structure) Cam followers Additional support at actuator point.		2
24	Rotation Mechanism	Align cartridges to actuators Align cartridges to fluid transfer positions Accuracy: .020 at 11" dia Torque: drag/stiction of alignment bearings Specific qty of stop positions or near infinite alignments?	Anti backlash Worm drives Direct gear drives Geneva mechanism No-backdrive DC motor, Stepper motor servo controller direct position sensing		2
25	Flex Line/Cable Rotation Trans.	+180/-180 motion maintain fitting/seals 2 high pressure (intake, exhaust) 1 low pressure (waste) Possibly 3+ AM handoffs? Cable bundle from controls	Snake pit, coil wrap torque coil	http://www.igus.com/wpck/default.aspx?Pagename=TwisterBand
26	Cartridge Actuators				4
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28	Cartridge Waste Collector				4
29	Waste Storage		On Cartridge No collector/flex lines, limited vol. Off Cartridge Higher Vol, req collector/flex lines		2
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31	TRANSFER TO AM
32	Cartridge to AM Selector	90+ ports in 3 ports out.	Mega-multi port valves. (90+ ports) Chip based valve to make multiport system Low pressure, robotic open/seal fittings.		5
33	AM Selector Injection Loop				4
34	Clean Injection Loop				4
35	Rotor Pass-Thru				2
36	LRAUV Platform Interface				2
37	LRAUV Pressure Housing	No tie rods thru housing Seal to tube section Maintain Structural support of nose/drive section	In house design Lock ring to tube	See model G3LRAUVHousing.sldasm	2
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Scored as follows:

1: COTS components, or process, readily available, used right out of the box, without modifications.
2: New designs, with known technology and variables (no prototype testing needed, can directly design deployment hardware.)
3: OEM components, or modified COTS.
4: New prototype and designs, with known theory/physics, but unknown technologies and variables. (prototypes need to be designed and tested prior to deployment hardware design.)
5: New design needed, but no theory/physics are yet known that would enable the technology.

Attachments:

Turbine Block Diagram (text/xml)

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